FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179149
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33090.28062611256083836No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31770.2694315985511585No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21240.1801299072466669No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT17960.15231323607109873No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.11576145169100768No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11990.10168350225459209No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC21400.051.2375532
GTGGTAT23050.047.221051
GTATCAA71400.036.7412221
TATCAAC80800.032.9264872
GGTATCA40300.032.705691
ATCAACG86300.030.8280433
AACGCAG86950.030.6953416
TCAACGC87200.030.5586034
CAACGCA89600.029.78755
ACGGTTG755.90626E-428.3329098
ACGCAGA99400.026.8079477
CGCAGAG100100.026.6204818
GCAGAGT107400.024.8110839
GTCGAGG11900.023.5710758
CAGAGTA59150.022.48909810-11
AGAGTAC56250.020.47524810-11
TCGAGGT13350.020.3742289
AGTCGAG18500.019.0672827
GAGTACG70700.018.60474212-13
TACGGGT14250.017.5962284
Produced by FastQC (version 0.11.5)