FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896640
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57560.3034840560148473No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44260.2333600472414375No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32940.1736755525560992No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23110.12184705584612789No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT23040.12147798211574153No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22250.050.640281
TGGTATC22800.049.2114372
GTATCAA87800.039.2741361
GGTATCA47050.037.32251
TATCAAC101350.034.6799742
TCAACGC104750.033.4731754
ATCAACG106150.033.1518173
CAACGCA107050.032.7539945
AACGCAG108600.032.402216
GACAGGT9600.030.1174321
ACGCAGA124150.028.4122417
CGCAGAG126100.027.9728768
GTCGAGG17650.027.4494888
GCAGAGT135000.026.0979449
TCGAGGT17850.024.9998059
AGTCGAG25200.024.4535337
CAGAGTA68850.023.45722610-11
AGAGTAC64400.021.67936310-11
GAGTACG81000.019.78123312-13
AGTACGG76100.018.12287312-13
Produced by FastQC (version 0.11.5)