FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896640
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40930.21580268263877173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT27600.043.14621
TGGTATC27600.043.1223032
GTATCAA106550.027.334191
TATCAAC116250.024.826732
AGAGTAC68250.024.66045211
CAGAGTA71300.023.87555110
ATCAACG124350.023.1420143
GGTATCA52800.023.1126293
TCAACGC124850.023.0053064
CAACGCA126850.022.7069955
AACGCAG128700.022.4666066
AGTACGG80350.020.67353613
CGATCGT804.4344884E-420.63137247
GAGTACG84550.019.93933112
ACGCAGA145050.019.8773297
CGCAGAG146900.019.6134648
GCAGAGT152750.018.8657919
GTACGGG92500.017.74991614
GACAGGT7800.017.6294651
GCCGTTA950.001410411917.36966115
Produced by FastQC (version 0.11.5)