FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768785
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23500.30567713990257356No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20970.2727680690960411No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14000.18210553015472467No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.15153781616446732No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8940.1162873885416599No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.10770241354865144No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA8010.1041903783242389No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13800.046.532041
TGGTATC13750.046.052712
GTATCAA48200.038.8203851
TATCAAC55100.034.939632
AACGCAG57300.033.155296
TCAACGC57750.032.9683844
GGTATCA24950.032.895841
ATCAACG58100.032.769783
CAACGCA58950.032.2972765
CTACGGA703.9279505E-430.3756921
ACGCAGA65550.028.9175877
CGCAGAG66600.028.4616788
GTCGAGG8700.028.334058
GCAGAGT71050.026.7423679
TGTCCGC808.6075737E-426.563178
TACGGAG850.001226469424.9990062
AGTCGAG12750.024.0006077
CAGAGTA36700.023.62764710-11
GGACCGT950.002342062522.3689867
AACCGAG1152.7220775E-422.174477
Produced by FastQC (version 0.11.5)