FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768785
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15550.20226721385042634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16000.035.397542
GTGGTAT16300.035.11081
ATATACG350.004088076831.4453891
GTATCAA58950.027.3513551
AGAGTAC36900.025.78627611
TATCAAC63100.025.314692
CAGAGTA37800.025.24506810
ATCAACG67600.023.6655923
TCAACGC67650.023.646564
AACGCAG67650.023.5668356
CAACGCA69250.023.1002145
ACCGTGT600.002038604622.9095368
GGTATCA28400.022.0892811
GACAGGT2650.021.8041151
GTACGGA1155.03469E-721.52344514
AGTACGG43850.021.51253913
GAGTACG44150.021.49094612
ACGCAGA76350.020.8814187
CGCAGAG76950.020.685578
GCAGTCG804.449763E-420.6185848
Produced by FastQC (version 0.11.5)