FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065927
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25490.2391345748817696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18950.042.6387372
GTGGTAT19750.040.789671
CGATCGT350.00409485931.43584447
CTATCGG400.00789829727.4818386
GTATCAA69850.026.8058971
CGGGCTA550.001225848325.00226217
AGAGTAC46650.024.46745911
GGTATCA35050.024.1483863
TATCAAC77600.023.9414882
CAGAGTA48000.023.7793110
ATCAACG82850.022.190093
TCAACGC82950.022.097084
CAACGCA83950.021.8993325
AACGCAG85450.021.6445646
GACAGGT4400.021.2459371
CGGTCCT650.003236133621.15774726
CGGGATT1951.8189894E-1221.15576217
CGGTGCG1055.5443325E-620.940517
TTAGACG1459.677024E-920.86395639
CGGGATA2900.020.86395617
Produced by FastQC (version 0.11.5)