FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558977
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91600.5875647940925364No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64900.030.3567491
GTGGTAT12600.028.6080111
TGGTATC12550.028.483532
TATCAAC74450.026.7034742
GGTATCA31300.026.5490821
TCAACGC76350.025.4169044
ATCAACG76600.025.373093
CAACGCA77400.025.1802565
AACGCAG78400.024.6845596
AGAGTAC74300.024.61166211
ACGCAGA79800.024.3203957
CAGAGTA76550.024.10070210
CGCAGAG81400.023.808588
ACGGGTC2500.023.10165816
GCAGAGT85000.022.7384539
AGTACGG85100.021.32869513
ACGGGGT10500.021.21580916
GAGTACG86700.020.9654612
CGGCTCG700.004979367419.64111528
TACGGGG33950.019.4417515
Produced by FastQC (version 0.11.5)