FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212308
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39780.3281344344836461No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32380.2670938408391267No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.19656720899309416No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT17050.140640827248521No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15190.12529819154868235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15550.056.027932
GTGGTAT16000.053.675211
GTATCAA64700.040.8722461
GGTATCA33850.037.679481
TATCAAC74050.036.272142
ATCAACG78500.034.2159463
TCAACGC78850.034.0640684
CAACGCA80900.033.4110225
AACGCAG82850.032.368166
GTCGAGG12100.029.8560688
CGCAGAG92100.029.2107778
ACGCAGA92850.028.9748277
GCAGAGT98350.027.397699
AGTCGAG15600.027.2442237
TCGAGGT12300.026.6063129
GACAGGT6850.025.4469431
CAGAGTA54800.024.46959510-11
GCGCTAT900.001707603623.6194551
AGAGTAC51950.021.76226410-11
GAGTACG64250.020.70561812-13
Produced by FastQC (version 0.11.5)