FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776632
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54090.30445246961666794No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52730.29679753601195974No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34260.19283678330684126No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.15788300559710733No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA26650.15000292688637828No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA21930.12343580437592028No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.11977719640308179No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT20960.11797603555491515No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC20640.11617487470674849No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT19510.10981452546166004No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT19100.10750678812494652No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC18520.10424218408764449No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG18380.10345417621657159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC37400.041.2461782
GTATCAA132100.038.847311
GTGGTAT38750.038.840791
AACGCAG148500.034.540576
TATCAAC153300.033.625392
TCAACGC155550.033.0570454
ATCAACG157050.032.768373
CAACGCA157550.032.664385
ACGCAGA164950.031.12177
CGCAGAG165500.031.0191448
GGTATCA71700.030.5975761
GCAGAGT177050.029.0932359
GACAGGT7550.028.1565591
CAGAGTA107900.025.13024910-11
GTCGAGG18400.024.0201878
GGCACCG7050.022.9076129
GCTTGTA10150.022.6195621
AAGTACG2655.456968E-1222.4614941
AGAGTAC105900.021.45108610-11
ACAGGTA9750.020.4950851
Produced by FastQC (version 0.11.5)