FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776632
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27360.15399925251824803No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA25920.14589402870149812No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT19730.11105282354477461No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT18890.10632477631833717No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC18660.1050301919587174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT41050.033.886231
TGGTATC41350.033.497042
GTATCAA146250.025.5452121
AGAGTAC108650.024.75658611
CAGAGTA110100.024.6803510
GCTTGTA8200.023.802931
GACAGGT6000.022.9089221
TATCAAC166200.022.3561532
AACGCAG166950.022.0240236
ATCAACG173100.021.3520683
TCAACGC172750.021.2356594
CAACGCA175600.020.9072385
GGTATCA78650.020.9020251
ACGCAGA186350.019.7634837
CGCAGAG187600.019.592818
TTGTACG3100.019.5018483
GGCACCG6750.019.148159
TAACGGC6350.019.05780236
GCAGAGT195650.018.8627649
GGCCGCG6300.018.77248640
Produced by FastQC (version 0.11.5)