FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254545
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT72950.5814857179296079No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50880.40556536433527696No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35520.2831305373661367No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT32040.2553913968809409No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13470.10736960411942179No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20000.057.167382
GTGGTAT20350.055.993151
GTATCAA68500.038.048231
GGTATCA39600.035.2162551
TATCAAC77800.033.543992
TCAACGC82750.031.6363774
ATCAACG83600.031.3147163
CAACGCA85450.030.7374445
AACGCAG87100.030.2527526
ACGCAGA95500.027.635187
CGCAGAG96700.027.1603938
GCAGAGT99700.026.3431329
CAGAGTA57200.024.5926910-11
GAGTACG63850.021.8640812-13
CCGGTCT1404.2029314E-521.2492739
AGAGTAC55850.020.54540310-11
GTACGGG72250.019.2338714-15
GTATAAC1400.00102274418.2216511
ACGGGAT12250.018.21293616-17
AGTACGG62800.018.16943412-13
Produced by FastQC (version 0.11.5)