FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254545
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64710.515804534711788No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13500.031.1695162
GTGGTAT13500.030.9843251
GTATCAA57100.027.711751
GGTATCA29100.025.72221
TATCAAC64900.024.6208922
TCAACGC67850.023.1782174
AGAGTAC62550.023.03508211
ATCAACG69100.022.7996253
AACGCAG69900.022.695126
CAACGCA69700.022.681355
CAGAGTA63550.022.54190610
CGCAGAG72800.021.7155258
ACGCAGA73350.021.627667
ACGGGTC1701.2551027E-1021.02965516
GCAGAGT76050.020.8253239
AGTACGG68950.020.10095413
GAGTACG70600.019.94118912
TGCCGAC8150.019.2364255
CGGGATA2650.018.6794617
GACGGAT1051.309158E-418.3291637
Produced by FastQC (version 0.11.5)