FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696310
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30200.4337148683775905No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20180.2898134451609197No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13530.1943100056009536No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA12520.1798049719234249No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT9470.13600264250118482No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC9020.12954000373396907No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT8610.12365182174606137No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT8260.11862532492711579No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG7900.1134552139133432No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA7330.10526920480820326No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC7230.10383306285993309No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA7230.10383306285993309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8450.052.303142
GTGGTAT8950.048.441981
GTATCAA32500.041.328381
GGTATCA16500.038.6413271
TATCAAC36400.036.8923952
TCAACGC39000.034.6508334
ATCAACG39100.034.453523
CAACGCA39750.034.103955
AACGCAG41000.033.3751456
ACGCAGA45650.029.9754857
CGCAGAG45550.029.85478
GCAGAGT48050.028.3898289
CAGAGTA29850.025.55472810-11
GACCCGA1001.05595886E-425.4977826
CTTAGAC1001.05595886E-425.4977823
GTATAGA1701.1876182E-825.0032121
GGGTTAC850.001225151525.0032121
GACAGGT2850.023.8627131
ACGGGAT5200.023.29124316-17
GAGTACG32850.023.02691512-13
Produced by FastQC (version 0.11.5)