FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498657
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT59100.39435307745534837No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40790.2721770224941398No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT33410.22293293261900488No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24800.16548149443134755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18700.052.5105932
GTGGTAT19200.051.371391
GTATCAA61300.040.086851
TATCAAC66850.036.6902772
TCAACGC72000.034.2384534
ATCAACG71900.034.108733
CAACGCA73900.033.3581625
GGTATCA35000.032.918181
AACGCAG76450.032.356696
ACGCAGA83250.029.7068027
CGCAGAG85650.028.8247748
GCAGAGT89150.027.6463839
CAGAGTA54150.024.60119610-11
GTACGGA1751.6158083E-824.293861
GAGTACG60400.021.95001412-13
AGCGTCA3350.020.2998943
AGAGTAC51700.020.21909510-11
GTATTAG6300.019.5700531
GTACGGG69400.018.91978314-15
ACGGGAT12250.018.38502316-17
Produced by FastQC (version 0.11.5)