FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854485
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.31984177604053904No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25060.2932760668706882No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14900.17437403816333816No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT14620.17109721060053718No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC11960.1399673487539278No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA10730.12557271338876635No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA10600.12405132916318015No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.12217885627015103No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT10090.1180828218166498No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC9550.11176322580267646No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC9080.1062628366794034No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT9050.10591174801196043No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT8900.10415630467474561No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA8760.10251789089334512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC23250.035.2782332
GTATCAA66900.035.1479721
GCTTGTA4650.032.0048941
AACGCAG73850.031.7083456
GACAGGT3500.031.5868681
GTGGTAT26150.031.3824541
ACAGCCG550.00443674930.9242041
TCAACGC79900.029.466974
TATCAAC81300.029.0640932
CAACGCA80950.029.0322555
ACAGGTA5050.028.6278551
GTCGAGG9100.028.4895978
ATCAACG82800.028.4349123
GCTTATA1054.5054676E-628.347191
TTGTACG1054.5239776E-628.332263
CGCAGAG84100.027.7948678
ACGCAGA85000.027.5489587
GCTTCGT808.585086E-426.575491
GCAGAGT89300.026.2239489
GACAGGA3100.026.0611271
Produced by FastQC (version 0.11.5)