FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016649
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49540.2456550445813823No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45890.22755571247153075No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA31210.15476168634204562No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27670.13720781355605263No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA25690.12738954572659894No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.12704243524777986No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC24590.12193495248801352No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT24340.12069527220651685No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC22960.11385223705265518No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT22550.11181916139100061No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21000.10413314364572118No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA20770.10299263778674424No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG20460.10145543423768835No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA20170.1000174051111522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC41500.040.143152
GTGGTAT43350.038.05391
GTATCAA136450.036.5806241
AACGCAG152600.032.8068286
TATCAAC158050.031.7293362
TCAACGC160150.031.2867434
CAACGCA162000.030.9294555
ATCAACG164350.030.6164973
GGTATCA79150.030.0273841
ACGCAGA169850.029.4749587
CGCAGAG170400.029.4054938
GTCGAGG17750.029.2109538
GCTTGTA10250.028.6208041
GACAGGT8400.027.3319761
GCAGAGT187700.026.69599
ACAGGTA10400.024.1198861
CAGAGTA115800.023.70960810-11
AGACAGG10500.022.2705021
ATTAGAG10900.021.8340853
CTTGAGT6500.021.5761572
Produced by FastQC (version 0.11.5)