FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016649
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34400.17058000673394327No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA29560.14657979648416755No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT23720.11762086510840508No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT22590.11201751023604009No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC21150.10487695181461919No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC20700.10264552730792519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC45450.031.7472572
GTGGTAT45900.031.6274071
GTATCAA153100.026.4706541
CGGGCCA1750.025.1457417
AGAGTAC118850.024.5995611
CAGAGTA120850.024.4421910
GCTTGTA9050.024.0005321
TATCAAC173650.023.234432
AACGCAG172500.023.1326836
TCAACGC177850.022.451714
CAACGCA180050.022.1926425
ATCAACG182000.022.0454623
ACAGCCG904.003966E-521.384441
AGACAGG6100.021.1841181
TCCGGAC1307.046401E-821.1426287
ACGCAGA191550.020.7916327
CGCAGAG192500.020.6640538
CTAGCGG804.4344953E-420.6314649
CGATCGG1351.04226274E-720.3732116
CCGGACT1351.0477379E-720.3631048
Produced by FastQC (version 0.11.5)