FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068159
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31240.1510522160046689No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30210.1460719412772422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73900.038.72161
GTGGTAT22650.038.2952231
TGGTATC22550.038.2570652
TATCAAC80900.035.563322
TCAACGC83900.034.443644
ATCAACG84000.034.2002683
CAACGCA85550.033.679985
AACGCAG86800.033.2928736
GGTATCA39500.031.5393831
ACGCAGA94700.030.4257897
CGCAGAG97300.029.6127668
GGTACGG1452.1882443E-929.3085353
GCAGAGT108500.026.5957769
CAGAGTA67800.022.62984810-11
CGGTCTA1455.51081E-520.5159748
AGAGTAC63550.019.99676510-11
ACGGGAC11200.018.9720443
GTACGGA1801.3470313E-518.8973181
GAGTACG79550.018.86055412-13
GTCGAGG10250.017.8281678
Produced by FastQC (version 0.11.5)