FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068159
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34290.16579963145967017No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT21350.10323190818500899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT25550.034.7603031
TGGTATC26650.032.696212
GTATCAA86000.025.9614831
TATCAAC95350.023.0912362
AGAGTAC68200.022.86357711
CAGAGTA71050.022.33244310
ATCAACG100600.021.8567393
TCAACGC100400.021.845534
AACGCAG102550.021.4684526
CAACGCA102300.021.4666615
GGTATCA46650.021.4546621
ACGCAGA114550.019.3399057
CGCAGAG116000.019.1274018
GCAGAGT123450.018.0425289
AGTACGG86050.017.89753313
GAGTACG87600.017.70600112
GCCGTTA1403.3224842E-617.6790915
GCTTGTA5150.017.084991
ACGGGAT12900.016.62839716
CGGGATT5500.016.50008817
Produced by FastQC (version 0.11.5)