FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419155
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT11750.28032589376245065No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT10840.25861554794765657No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT10520.25098114062816856No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT10330.24644821128222255No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA8320.19849459030668845No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC8280.19754028939175244No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGA7670.18298720043897843No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA7490.1786928463217664No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGATA7410.1767842444918944No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAAAAGA7380.1760685188056924No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTG7260.1732056160608844No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6840.16318545645405635No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTA6770.16151542985291836No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA6740.16079970416671638No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG6630.15817537665064235No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTTTCACTTCC6570.15674392527823836No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC6520.15555104913456835No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT6210.14815521704381435No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCC6140.14648519044267633No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGCGAGCGAACGGG5890.14052080972432632No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATTGATTTCTTTTC5880.14028223449559232No Hit
GGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCT5560.1326478271761043No Hit
TCGCATGACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCA5490.1309778005749663No Hit
GTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAAAAGAAATCAAT5360.12787632260142429No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATTGATT5280.12596772077155227No Hit
GTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCT5120.12215051711180827No Hit
GTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGG5010.11952618959573429No Hit
GCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAAC4740.11308465841991626No Hit
GTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGA4730.11284608319118226No Hit
CTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTTTCACTTCCCCT4670.11141463181877827No Hit
GTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGA4620.11022175567510825No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTT4570.10902887953143825No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA4500.10735885293030026No Hit
CCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGAT4440.10592740155789623No Hit
TCCTAAGGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATC4420.10545025110042824No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCATAAGGTAGGTA4410.10521167587169425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGT1000.035.780041
ATCGTAC401.8994418E-434.3751546
GTATCAA25700.033.1990781
TGGTATC3850.032.1429982
GTCTAAT350.004079879331.4549771
ATGCTAG453.7913732E-430.55568715
ACTATCG1900.030.39124311
GGTATCA10500.030.1443561
TATCAAC29100.030.0516782
GTACTGG2250.029.3579831
ATCAACG30400.028.3108883
CTATCGG2050.028.17085512
GGTCAGT2050.028.17085517
TCAACGC30900.028.0307584
AACGCAG30650.027.9902556
CAACGCA30900.027.7604855
AGAGTAC30650.027.63140711
CGCAGAG30900.027.5858258
CGGGCAA1004.298272E-927.5001217
TGCTAGA507.024236E-427.5001216
Produced by FastQC (version 0.11.5)