FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005456
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23390.23263076653776993No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAG15070.14988224248500184No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAATCATCAAGGAT12750.1268081348164415No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11850.11785697235881032No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10210.10154596521379355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC27450.044.151522
GTGGTAT27750.043.8962941
CGGGCAC1500.027.50121917
GTATCAA108300.027.4463351
AGAGTAC62900.026.40816511
CAGAGTA65150.025.41171810
GCCGACG550.001225774525.00235255
TATCAAC119400.024.7891032
GGTATCA52500.023.9736423
ATCAACG128900.022.9396723
TCAACGC129150.022.8739874
GACAGGT4250.022.6446821
CAACGCA131350.022.511795
CGGGATA4400.022.50099817
AGTACGG73550.022.17297413
AACGCAG134150.022.1454476
GAGTACG73850.022.04566412
CGGGGCC1658.367351E-1121.66762517
CGGGACA2600.021.15478317
CGGTCGT650.003238637221.15478313
Produced by FastQC (version 0.11.5)