FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769780
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54990.7143599469978436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6550.034.401822
GTGGTAT6700.033.2322851
GTATCAA28450.028.8894231
ACGAGTC400.00789773227.4812875
TATCAAC31750.025.7917842
GGTATCA13400.024.8216441
AGAGTAC29700.024.807611
CAGAGTA30350.024.27314610
TCAACGC34250.024.066514
ATCAACG34800.023.7651023
CAACGCA35050.023.5217865
AACGCAG35300.023.4345746
ACGCAGA36350.022.6820417
CGCAGAG36800.022.404688
AGTACGG33500.021.51099613
GCAGAGT38250.021.3439589
CGGGATA1558.967618E-1021.29520417
TAATACG650.003255274121.1353344
GAGTACG35050.020.71128312
ACGGGAT7600.020.26780516
Produced by FastQC (version 0.11.5)