FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134650
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT96930.854272242541753No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT69390.6115542237694443No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49840.43925439562860796No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32420.28572687612920283No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT16830.14832767813863307Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT14410.12699951526902567Illumina PCR Primer Index 10 (95% over 23bp)
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12640.11139999118670955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT17950.057.545261
TGGTATC17900.057.2234882
GTATCAA61900.038.6621061
GGTATCA33750.037.1548921
TATCAAC69550.034.893882
ATCAACG74950.032.3217163
TCAACGC75300.032.171484
CAACGCA77250.031.4144025
AACGCAG80500.030.146126
CGCAGAG89950.026.975458
ACGCAGA91250.026.637717
CAGAGTA47350.026.29549610-11
GCAGAGT93800.026.140079
TATACCG900.001710999523.6112355
GAGTACG53650.022.97005712-13
AGAGTAC45800.022.22152110-11
GTATTAG3550.021.5532071
GTACGGG57850.021.1554814-15
GATCGAC3100.020.56190520-21
TCGACAC3050.020.20324322-23
Produced by FastQC (version 0.11.5)