FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015735
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44390.22021743929633608No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42400.21034510984826874No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27980.1388079286215698No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT23970.11891444063827834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT27300.048.276331
TGGTATC29500.044.2272532
GTATCAA89100.038.2199441
TATCAAC101450.033.9736632
TCAACGC106350.032.4882744
ATCAACG107800.032.0512773
AACGCAG108400.031.756266
CAACGCA108700.031.7468075
GGTATCA51150.031.335091
ACGCAGA123750.027.8515427
CGCAGAG126450.027.2239158
GTCGAGG14800.026.1314838
GCAGAGT135750.025.5160189
GGTACGG1302.3729874E-522.8838083
CAGAGTA75200.022.57844710-11
AGTCGAG22150.021.680957
TCGAGGT18650.020.2817929
AGTACGG92450.019.9046522
GCTTGTA9450.019.7950251
AGAGTAC72450.019.68109710-11
Produced by FastQC (version 0.11.5)