FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015735
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27470.1362778341399043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT33900.037.1401631
TGGTATC33750.036.8869822
GTATCAA113500.025.9643211
AGAGTAC83950.023.5887811
TATCAAC125550.023.3340022
CAGAGTA86200.023.06878310
TAGCGCG600.002035278122.91817734
GGTATCA60900.022.208841
TCAACGC132050.022.0386354
ATCAACG132650.021.9803853
AACGCAG134950.021.6679346
CAACGCA135400.021.5542565
TACGCGA903.9962833E-521.38976729
ACGCAGA150550.019.4246277
CGCAGAG152100.019.1762738
AGTACGG102900.019.00459713
GAGTACG104200.018.9258712
GCAGAGT162200.018.0393039
GTATTAG5900.017.7055591
ACGGGGC9550.017.27991316
Produced by FastQC (version 0.11.5)