FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775461
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26340.148355835470337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC41350.039.957942
GTGGTAT41650.039.6219751
GTATCAA138950.025.0002691
AGAGTAC86700.023.2824111
CAGAGTA89000.022.74252710
TATCAAC153500.022.6740672
GGTATCA75500.022.4272843
ATCAACG164550.021.0649363
TCAACGC164350.020.9896284
AACGCAG168650.020.553416
CAACGCA169400.020.4288085
AGTACGG105650.018.79397413
GAGTACG106100.018.76610412
ACGCAGA188250.018.386847
CGCAGAG189300.018.2573498
GCAGAGT196200.017.6593029
TATACGG1252.4940238E-517.5918165
ACGGGAT12800.016.9733716
TACGGGC13800.016.5405515
CGGGGTC7050.016.38366717
Produced by FastQC (version 0.11.5)