FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617486
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10970.17765584968728038No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA6800.11012395422730231No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6540.10591333244802312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG903.8198777E-1133.584043
GACAGTA852.7068381E-829.1154161
CGCGGTA2100.026.18846943
GCTTGTC858.788229E-725.880371
GCGGTAC2250.025.66470144
CTTGTAC1500.025.6480732
GTGGTAT27450.024.7430951
CCGCGGT2250.024.44455342
GCTTATA801.6076177E-524.0606581
ACAGTAT801.6157068E-524.0450692
TGGTATC28200.023.9719812
TAACGGC2300.023.91508736
GCTTGTA3000.023.8315071
AGAGTAC48050.023.81056811
GACAGGT2200.023.7481781
CAGAGTA48700.023.54923810
GTCGACA952.311377E-623.15987414
GACAGCC952.3146367E-623.156121
ACAGACG952.3146367E-623.156121
GTATCAA78350.023.1284121
Produced by FastQC (version 0.11.5)