FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720842
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14400.19976638431167998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12900.043.8914642
GTGGTAT13550.042.0062981
ATCGTAG507.0197636E-427.50642449
GTACCGA400.00789427327.483526
GTATCAA46450.024.6850321
CGGGGCG701.79856E-423.5736617
GGTATCA25250.023.3042831
AGAGTAC31400.022.1597411
TATCAAC53050.021.6567552
CAGAGTA32450.021.44121710
ATCAACG57050.020.0887453
TCAACGC57150.020.0055034
AGTACGG35150.019.71737113
GTCTTAG700.004977534519.6406251
CGTATAA700.00497953319.63926330
CAACGCA58400.019.6243655
CTGTTCG856.6856964E-419.409569
GAGTACG35550.019.3407912
AACGCAG59400.019.2477196
TACGGGG13200.019.16848215
Produced by FastQC (version 0.11.5)