FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584771
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39850.25145588857948564No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38000.2397822776918558No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25040.15800390087905444No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT21980.1386951174649208No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.10051925483240166No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC26700.047.907752
GTGGTAT26450.047.9057771
GTATCAA88750.036.411831
GGTATCA50350.033.1887051
TATCAAC101450.032.547642
TCAACGC104300.031.6175334
ATCAACG104750.031.5628433
AACGCAG105300.031.47876
CAACGCA106200.031.1719155
GTCGAGG13350.029.6069288
ACGCAGA118500.028.0439367
CGCAGAG120550.027.4991368
GCAGAGT130250.025.4520199
AGTCGAG22750.023.3495867
TCGAGGT16850.022.9533929
CAGAGTA76900.022.49541710-11
TAGAGCG1404.2055814E-521.2481235
AGAGTAC73500.020.00850710-11
TGGACCG1507.152953E-519.8315815
AAGTACG2252.2999848E-718.8979531
Produced by FastQC (version 0.11.5)