FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584771
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21540.1359186910916467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC28900.037.6533362
GTGGTAT29950.036.809451
GTATCAA99100.024.6071741
GGTATCA54200.022.72431
AGAGTAC70500.022.00290511
TATCAAC111200.021.9192122
CAGAGTA73550.021.24005910
ATCAACG119750.020.2394733
TCAACGC119950.020.182824
AACGCAG122200.019.882436
CAACGCA122800.019.7367845
GCTTGTA4900.018.5152511
CGATCGT750.00740204918.33807247
ACGCAGA136450.017.8474437
GTATTAC2800.017.6736491
CGCAGAG137750.017.5820148
AGTACGG88200.017.55617313
CGGGCTA950.001416276217.3586526
GAGTACG89500.017.33189812
TTAAACG2102.1045707E-917.02659622
Produced by FastQC (version 0.11.5)