FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076352
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20750.19278080033297657No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18560.1724342965869901No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.12282227375431086No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT10890.10117508027113807No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT15100.043.0718841
TGGTATC16700.038.934382
GTATCAA48000.036.6638831
TATCAAC53800.033.334082
AACGCAG57200.031.5012746
TCAACGC58150.030.8404734
ATCAACG57900.030.8268433
CAACGCA59150.030.3909245
GGTATCA28200.028.4899751
ACGCAGA63400.028.4207087
CGCAGAG66300.027.1134728
GCAGAGT70900.025.4142889
GTCGAGG7550.024.7664688
CAGAGTA42850.021.57082610-11
TCGAGGT8950.021.367219
AGTCGAG11450.020.7845617
AGAGTAC41750.019.54353310-11
AGTACGG51600.019.4366132
GAGTACG53250.018.9194321
GCTTGTA3401.0913936E-1118.7539041
Produced by FastQC (version 0.11.5)