FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076352
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16550.15376010821738612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18050.037.0138661
TGGTATC18750.035.030832
TATACCG400.00790120527.4797925
GTATCAA57100.026.4345341
TATCAAC63250.023.7238882
AGAGTAC43400.023.00562511
CAGAGTA43750.022.8215810
GGTATCA29900.022.6087913
AACGCAG67400.022.1000466
TCAACGC67600.021.8700124
ATCAACG68250.021.7825163
CAACGCA68850.021.5527785
CCGATTC650.00323596721.15795914
CGGGCTA650.00323596721.15795917
GCTTGTA3550.020.1363351
ACGCAGA75650.019.7290087
CCGCTAT856.672691E-419.41644325
ATACCGT1009.0318266E-519.2376426
CGCAGAG78250.019.1490658
ACGGGAT7400.018.58469416
Produced by FastQC (version 0.11.5)