FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544093
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16790.30858695112784024No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15680.28818602702111584No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.16449393761728234No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.16210464019937768No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC7890.14501197405590588No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC7160.13159515009382586No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7060.12975722900313No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA6920.12718413947615573No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA6790.12479484205825107No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.12111899987685928No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC6490.11928107878616338No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA6470.11891349456802422No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT6320.11615661293198037No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA5750.1056804627150138No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT5720.10512908638780503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12750.039.9951132
GTATCAA43750.037.5029031
GTGGTAT13700.036.611461
AACGCAG49000.034.256026
TATCAAC51950.032.147182
TCAACGC52200.031.9932174
ACTGCGC802.3040087E-531.8769658
ATCAACG52650.031.8004823
CAACGCA53100.031.5309875
ACGCAGA55300.030.3534367
CGCAGAG55950.030.0063198
GCAGAGT59700.028.1952849
GGTATCA23900.028.100551
GCTTGTA3650.026.7849331
GTCGAGG6350.026.7733048
AGACTGA808.611735E-426.5592566
AGACAGG2650.025.6643181
CGGGTAT850.001226597324.9969466
CAGAGTA35250.024.65981510-11
GACAGGT2950.024.4952851
Produced by FastQC (version 0.11.5)