FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544093
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9630.1769918010340144No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC7290.13398444751173053No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG7100.13049239743940833No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA6590.12111899987685928No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTT6530.12001624722244177No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT6010.11045905755082311No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT5820.10696700747850091No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT5510.10126945209734364No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT5450.10016669944292611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTT901.3915269E-930.56532151
TGGTATC15250.030.4599232
TGCTTAA1450.030.34056319
GTGGTAT16000.029.5583441
GACAGGT1251.8189894E-1228.5959841
CTGCTTA1550.028.3857218
ACAGGAG1550.028.3831081
CTTGTAC1256.184564E-1126.3865832
TTGTACG958.009374E-826.0346033
TCTACGC858.754687E-725.89062547
GTATCAA50700.025.4352841
AGAGTAC35700.025.1924911
CAGAGTA37000.024.6790210
GACACAT2900.023.71447426
GGCCGCG2900.023.71010840
CAGATCT1052.0952575E-723.57028641
ACCACTG2250.023.22536814
CTTAAAC1900.023.16103421
TGACACA3100.023.07188625
TACCACT2150.023.02637713
Produced by FastQC (version 0.11.5)