FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159089
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19270.16625125421775205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22050.036.263372
GTGGTAT22700.035.4839251
GTATCAA76450.027.4370171
AGAGTAC60200.024.85412811
CAGAGTA60250.024.78785110
TATCAAC85600.024.3641762
ATCAACG91250.022.7652653
AACGCAG91700.022.7173966
TCAACGC91000.022.6768254
CAACGCA92400.022.4224515
GGTATCA38550.021.953823
TTGTACG904.0204948E-521.371713
ACGCAGA103150.020.1965587
CGCAGAG104300.019.9816348
GACAGGT4050.019.6848831
AGTACGG74450.019.6536413
GAGTACG75800.019.63017512
GTATAGA1702.8594513E-919.4053821
CGGGAAA8700.019.28442617
GCTTGTA4150.019.2105481
Produced by FastQC (version 0.11.5)