FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064374
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16670.15661788055702225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22350.039.8432582
GTGGTAT23350.037.6731641
TCGACCG400.00786929427.50256534
GTATCAA72900.023.681061
GGTATCA42100.022.0006333
GGTCGAC752.8788796E-421.9927448
AGAGTAC53350.021.34319911
TATCAAC81200.021.121072
CAGAGTA54800.021.0294110
CGGGCTG1108.240302E-620.00280417
ATCAACG86950.019.7243353
TCAACGC87250.019.5935134
AACGCAG88500.019.38076
CAACGCA89250.019.3084165
TTAAACG1805.7734724E-918.33676522
CGGGATA2850.018.33590317
ACTATCG1706.0003E-817.79661211
ACGCAGA97800.017.5675077
CGCAGAG98500.017.4434818
GTACTAT1606.2438085E-717.1810231
Produced by FastQC (version 0.11.5)