FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808621
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57000.7049037806339434No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAG9850.12181231998674286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16800.033.2301752
GTGGTAT17350.032.1927031
GTATCAA80100.028.8541051
TATCAAC90850.025.8510862
AGAGTAC78300.025.67129311
CAGAGTA78600.025.57331310
GGTATCA37450.025.3470921
GACAGGT2400.025.2215821
GGATGTC550.001226022725.0007619
AACGCAG93300.024.9861266
TCAACGC95000.024.5085534
ATCAACG95500.024.3529573
CAACGCA96900.024.114595
ACGCAGA97550.023.9257267
CGCAGAG97150.023.6846738
CGGGCCA701.7996096E-423.57214417
GCAGAGT99000.023.247839
GCTTGTA1950.022.5759621
CGGGATA3300.022.50068317
GAGTACG88950.022.31944512
Produced by FastQC (version 0.11.5)