FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389047
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12970.33337874344230906No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12290.31590013545921186No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7490.19252172616676136No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.1750431181836642No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.12106506411821708No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT4640.11926579564936884No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC4080.10487164789858294No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC3910.10050199590280866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG603.2041917E-642.4983943
TGGTATC8550.039.7645842
GTGGTAT8600.039.0542831
ACAGGTA2500.035.712421
GTATCAA29200.035.234861
CTTGAGT1850.034.4581572
GACAGGT1650.033.49651
AGCGTCA652.5508314E-432.6910743
GCTTGAG2000.031.8860911
AAGTACG952.0641364E-631.3266871
TATCAAC33850.031.1361922
GGTATCA16600.030.9896951
ATCAACG34800.030.5304533
TCAACGC34700.030.4959664
CAGGTAT2250.030.221082
GCTTGTA2400.030.1146411
AACGCAG35150.029.9846386
CAACGCA36100.029.3132955
GTGCGCT755.899874E-428.332269
GTCGAGG4450.027.6955838
Produced by FastQC (version 0.11.5)