FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389047
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6360.16347639231249694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCCA300.001921053536.67833325
TGGTATC10650.035.0924152
GTGGTAT10850.034.95661
CGTTGCG350.004100267331.422444
CGCGGCC901.3915269E-930.56134611
GCTTGTA1201.8189894E-1229.7743231
CTTGTAC653.2162789E-629.594342
CCGTTAA1004.287358E-927.50521316
CGCTCGT400.00785955827.5052140
TTGTACG606.337799E-527.4804573
CTTGAGT1103.965397E-1027.4804572
GGCCGTT1057.214112E-926.19543814
GTATCAA37500.026.0181791
AGAGTAC25750.025.31547511
CAGAGTA26250.025.14762310
CCCGGCC550.00122282325.00795624
CAGGTAT1601.8189894E-1224.0484921
TATCAAC41050.023.698132
GACAGGA1653.6379788E-1223.319751
TCCGAGC600.00203088222.9210111
Produced by FastQC (version 0.11.5)