FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460221
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39990.16254637286650264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT34550.037.9609871
TGGTATC34500.037.4459342
GTATCAA118150.025.1570171
GGTATCA65050.023.2901081
TATCAAC132300.022.3967382
AGAGTAC90600.022.0987411
CAGAGTA94100.021.39369410
ATCAACG141100.020.8610123
TCAACGC141350.020.7657874
CAACGCA144450.020.3962425
AACGCAG145550.020.2991586
CGCGTAG1151.2110695E-519.1322234
ACGCGTA1151.2129833E-519.12871633
ACGCAGA162200.018.2342247
CGCAGAG162400.018.1657948
GCAGAGT170400.017.3480329
AGTACGG113950.017.3293913
GCTTGTA7550.017.1166231
GAGTACG116150.017.09586712
GTCGAGG15200.015.3750318
Produced by FastQC (version 0.11.5)