FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441285
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24830.17227682241888315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24700.042.8313752
GTGGTAT24950.042.3082161
GTATCAA96000.027.5465931
CCGACCG400.00787936527.4960069
AGAGTAC64650.026.67314311
CAGAGTA66300.026.0913910
TATCAAC103350.025.3118972
ATCAACG112250.023.2772733
TCAACGC112750.023.1496814
GGTATCA47250.023.1429793
CAACGCA114850.022.797375
AGTACGG76050.022.56709513
AACGCAG116150.022.566656
GAGTACG76650.022.42632912
ACTGCGA1155.0350536E-721.52608924
ACGGGAT15600.020.8040416
CGGGATA4250.020.7086317
TACGGGC11450.020.17734715
CGCAGAG130200.020.0356868
ACGCAGA130900.019.983917
Produced by FastQC (version 0.11.5)