FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378933
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49140.356362491868713No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41570.30146497328006505No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30090.2182121974019042No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT22380.16229940105864463No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA19800.14358928243794294No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18150.13162350890144772No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC17630.12785247724146132No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT16310.11827985841226514No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15480.11226071172420996No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG15300.11095535461113773No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA14180.102833132574244No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT14120.10239801353655327No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA13840.10036745802732984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24800.050.3823552
GTGGTAT25550.049.0911181
GTATCAA88450.038.7444041
TATCAAC99650.034.8866122
GGTATCA49250.034.7912981
TCAACGC107450.032.275024
ATCAACG108000.032.0319563
AACGCAG108550.031.908816
CAACGCA109000.031.7770755
GTCGAGG13350.029.2879758
ACGCAGA121700.028.4260587
CGGGCGA755.9067394E-428.3329356
CGCAGAG125250.027.620378
GCAGAGT131200.026.336339
CAGAGTA68750.024.26417210-11
AGTCGAG21850.023.9241497
TCGAGGT16750.023.0900639
GACAGGT6100.023.0014881
AGAGTAC67000.020.55269410-11
GAGTACG81100.020.38577812-13
Produced by FastQC (version 0.11.5)