FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121906
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25160.22426121261496063No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21220.1891424058700105No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15050.1341467110435277No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT13760.12264842152551106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16900.044.5122532
GTGGTAT17700.043.24581
GTATCAA57900.038.0448571
TATCAAC65650.033.5989462
TCAACGC68050.032.4764254
ATCAACG68600.032.1540953
CAACGCA69650.031.730385
AACGCAG70050.031.6084566
GGTATCA33900.031.360651
GTCGAGG7000.028.5359848
ACGCAGA77750.028.47817
CGCAGAG78600.028.1713878
GCAGAGT85750.025.8719659
CAGAGTA52350.022.20610210-11
AGTCGAG13350.020.6921567
AGCGTCA2101.1168413E-720.2382873
GACAGGT5700.019.3973871
AGTACGG65850.019.1042042
TCGAGGT9350.019.091099
AGAGTAC50550.019.08694610-11
Produced by FastQC (version 0.11.5)