FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121906
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17270.15393446509778894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19600.039.274062
GTGGTAT20600.038.0500951
ATCGTCG401.9008457E-434.37706440
GTATCAA67900.025.6801511
TCGTCGC550.001226106825.00150141
TATCAAC74150.023.4320222
AGAGTAC50400.022.80789211
CGATACG852.5672874E-522.65043625
CAGAGTA52100.022.22203310
CGTACGA752.8714328E-422.0013235
ACGTACG752.8714328E-422.0013234
TCAACGC78400.021.9825384
ATCAACG78700.021.8987453
AACGCAG78900.021.8442086
CAACGCA79500.021.6092285
GGTATCA36850.021.5569233
CGATAGG903.9922925E-521.39112722
CGCGTTA650.003241087321.15228720
TAACGGC1055.514754E-620.95363836
CGAAACG804.4470688E-420.6216430
Produced by FastQC (version 0.11.5)