FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064086
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13680.1285610373597623No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT13570.12752728632836066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8500.032.7060471
TGGTATC8750.031.1102432
GTATCAA41100.029.8018911
GGTATCA19750.028.152041
TATCAAC47400.026.1042022
TCAACGC48450.025.246414
ATCAACG49100.025.0265063
AGAGTAC47950.024.77375411
AACGCAG49050.024.7729596
CAACGCA49800.024.728775
CAGAGTA49950.023.89191210
ACGCAGA51500.023.594447
CGCAGAG52000.023.5283838
GCGGATT952.318331E-623.15488624
GTCTAAG1103.2579192E-722.5203671
GCAGAGT54800.022.2781289
AGTACGG53450.021.66067113
GAGTACG54550.021.37411712
TACGGGT10850.021.2905615
TTAACGG1351.04097126E-720.3734735
Produced by FastQC (version 0.11.5)