FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736881
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14200.1927041136899988No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA10630.1442566710228653No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT8490.11521534684704857No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT7810.10598726252949935No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC7380.10015185626987262No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT7380.10015185626987262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGT2500.030.79271
TGGTATC31800.027.6570072
CTGCTTA1850.026.7576818
GTGGTAT33200.026.4997431
TTGTACG1201.0932126E-925.1920973
CGGGCGA550.00123026424.9855926
CCCTTGC1251.7389539E-924.20412453
GCTTGTA3300.023.3278031
CAGACGC952.3257307E-623.1445482
AGACGCA952.3271023E-623.1429793
GTATCAA90600.023.0022721
AGAGTAC58950.022.9073511
CAGAGTA59850.022.8845510
TGCTTAA2300.021.52102319
AACGCAG97050.021.466246
GTGTATA1055.5247074E-620.9474141
TATCAAC99300.020.8691332
ACTTCGC1207.5772186E-720.62711510
CGGGAAA7900.020.19194217
GCTTGTC1108.261548E-619.9952581
Produced by FastQC (version 0.11.5)