FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631975
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12320.19494442026978914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10900.043.3820082
GTGGTAT11100.043.1093521
GTATCAA41250.025.7340741
GGTATCA21150.024.7052021
AGAGTAC26800.023.60329211
TATCAAC45050.023.5559272
CGGGATA1653.6379788E-1223.33585217
CAGAGTA28450.022.524410
ATCAACG48600.021.6621463
TCAACGC49000.021.5414094
CAACGCA49700.021.1827015
AACGCAG50450.020.922286
CGGGAAC2001.8189894E-1220.62722617
AGTACGG30300.020.42299813
GAGTACG31300.020.03411112
ACGGGAT6450.019.6145216
CGCTCCG1702.8558134E-919.4031075
ACGCAGA55700.018.9502537
CGCAGAG55900.018.885448
GTACGGG33200.018.39054114
Produced by FastQC (version 0.11.5)