FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077268
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46850.22553661828902194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT39250.043.15541
TGGTATC39750.042.5208552
CGCGCGC400.00786873527.50417940
ACCGGTC507.0474116E-427.4909348
GTATCAA157600.027.2532881
AGAGTAC87850.026.04772611
CAGAGTA93100.024.81520310
TATCAAC172400.024.7888952
GGTATCA76200.024.0693821
ATCAACG184500.022.9689713
TCAACGC185000.022.8474734
CAACGCA188450.022.4146145
CGGGCGA752.8721898E-422.00175517
AACGCAG192350.021.9892526
AGTACGG103800.021.78024313
GAGTACG105750.021.50866112
CGGGGTA2850.020.26477417
TACGGGG39600.019.93310415
ACGGGAT20450.019.90426616
CCGACGA700.00496883219.64868250
Produced by FastQC (version 0.11.5)