FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917669
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30650.3339984242684454No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26960.29378784725211377No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18510.20170671560224873No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT13830.15070793499616963No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11880.12945844307697002No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12950.053.4934162
GTGGTAT13900.049.8618471
GTATCAA49850.039.151021
GGTATCA26550.035.873941
TATCAAC56950.034.5517272
TCAACGC60750.032.390474
ATCAACG61250.032.264833
CAACGCA61550.031.9694735
AACGCAG64300.030.4700016
GGCGTAC600.00680536828.3329166
CGCAGAG70950.027.5542098
ACGCAGA71450.027.361397
GTCGAGG8250.026.7874858
GCAGAGT74600.026.2644489
GACAGGT5550.024.5161651
CAGAGTA39450.024.29437610-11
TCGAGGT9300.022.3933649
GAGTACG44350.021.37063612-13
AGAGTAC36850.021.33734910-11
ACGGGAT8700.020.51694116-17
Produced by FastQC (version 0.11.5)