FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016441
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18070.17777716562004092No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA14620.1438352053882124No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT11040.10861427274185122No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT10420.10251455814946465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG300.00193042236.6476753
TGGTATC34350.030.3293362
GTGGTAT35150.029.8795431
CGTACGA606.3118176E-527.5033535
ACGTACG507.026351E-427.5033534
GGATCTC759.760395E-625.66347519
GTATCAA108400.025.4140781
GCTTGTA4200.024.8754081
CTGCTTA2900.023.70628218
AGAGTAC74200.023.46425411
TTGTATC2000.023.3628923
CAGAGTA75350.023.36165810
GCTTGTC1553.6379788E-1123.0593781
TATCAAC120450.022.8671112
TGTATCA2050.022.7930644
AACGCAG121200.022.6099836
CTTGTAT3200.022.3343732
CGGGCAC1003.6107867E-622.0026817
CGGGCAA2500.022.00267817
CTTGCGG1505.984475E-1022.00051155
Produced by FastQC (version 0.11.5)